Micro Rheometer is Latest Lab On a Chip Device

Researchers at the National Institute of Standards and Technology
(NIST) have demonstrated a microminiaturized device that can make
complex viscosity measurements—critical data for a wide variety of
fields dealing with things that have to flow—on sample sizes as small as
a few nanoliters. Currently a table-top prototype, the NIST rheometer
could be a particularly valuable tool for biotechnologists studying
minute quantities of complex materials that must function in confined
spaces.

The NIST MEMS-based rheometer (click to retrieve mpg file of the device in action.) The moving plate is controlled by resistance heating elements in the chevron-like structure at the top; expansion and contraction of the vanes causes the plate to move up and down. Central square where the sample would rest is approximately 500 micrometers across. Credit: Christopher/NIST View hi-resolution image

Viscosity, elasticity and how materials flow when subject to a force
is the subject of rheology, and the measurements tell a lot about a
complicated material like a gel. Is it more like a liquid or a solid? By
how much and under what conditions? The popular toy Silly Putty™ is a
classic example of complex viscoelasticity, bouncing better than a
rubber ball under a sharp, sudden force but slumping into a puddle when
left alone.

One common way to make dynamic rheology measurements (how behavior
changes with the speed or frequency of the applied force) is with a
sizeable lab instrument that traps a test sample between a fixed plate
and one that moves, and measures how much the thin layer of test
material resists being deformed. Typical sample sizes are around a
couple of milliliters, which doesn't sound like much, but, says polymer scientist Gordon Christopher, for some researchers it's a whole bunch.

"A lot of people in the biosciences are making very complex designer
fluids based on proteins where you might make only 10 milliliters at a
time. Polypeptide hydrogels for drug delivery or tissue replacement, for
example," Christopher explains. "Their flow behaviors are very
complicated and you really need to understand them, but in a traditional
rheometer your sample for a single test is a large percentage of what
you just spent two months making."

Inspired by a talk by a NIST scientist working on the design of novel
nano positioning microelectromechanical systems (MEMS), team leader
Kalman Migler and his colleagues began a collaboration to build a MEMS
device that duplicated a classic sliding-plate dynamic rheometer—but in a
space about one-twentieth the size of a postage stamp. The sample size
of the MEMS rheometer is about 5 nanoliters. "With our device, if you gave me a milliliter of sample, I could give you back hundreds of tests," Christopher says.

Equally as important, he says, the MEMS rheometer inherently tests
materials when they are confined in a very small space. For many
biological applications where the material is meant to be used in a
confined region like a blood vessel or the interior of a cell—or must be
injected through a thin needle—understanding the flow characteristics
of small amounts in a small space is more important than knowing how it
behaves in bulk.

NIST's early prototype MEMS rheometers include only the core sliding
plate mechanism on the MEMS chip, and rely on a microscope and
high-speed cameras for the actual measurements. In a more polished
version, according to the research team, the necessary sensors could be
included on the chip and the entire instrument reduced to a handheld
device for, e.g., quality control measurements on a plant floor. The
NIST MEMS dynamic rheometer is described in a new paper in Lab on a Chip.*

Media Contact: Michael Baum, baum@nist.gov, 301-975-2763